Status
[June 2021] The Saccharina latissima SL-CT1-FG3 v1.0 genome was sequenced with PacBio and assembled with HiFiAsm by the HudsonAlpha Genome Sequencing Center. The resulting assembly was polished using RACON, scaffolded using HiC data, and screened for contaminants. Aquatic genomes can often harbor contaminants that are difficult to identify, so Illumina depth was employed to assist in contaminant screening. The transcriptome was sequenced with Illumina by HudsonAlpha, and then assembled with Trinity by the Joint Genome Institute (JGI). Subsequently, the JGI Annotation Pipeline was used to generate structural and functional annotations. Additional screening of contaminants using NCBI's FCS_GX and vector screening identified an additional 218 gene models that were excluded from FilteredModels3 to produce the default analysis track ("FrozenGeneCatalog_20240409"). The mitochondrial and plastid genomes were assembled separately, annotated by the Nuzhdin lab using the GeSeq MPI-MP Chlorobox program, and are available in the Downloads page.
Genome Assembly | |
Genome Assembly size (Mbp) | 615.55 |
Sequencing read coverage depth | 185.23x |
# of contigs | 1513 |
# of scaffolds | 1180 |
# of scaffolds >= 2Kbp | 1180 |
Scaffold N50 | 109 |
Scaffold L50 (Mbp) | 1.35 |
# of gaps | 333 |
% of scaffold length in gaps | 0.5% |
Three largest Scaffolds (Mbp) | 11.32, 8.81, 7.48 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
Ests | est.fasta | 571770431 | 443028122 | 77.5% |
Other | JGI_RNA_contigs | 503924 | 120242 | 23.9% |
- Please note: Using BLAST, unmapped RNA contigs were found to hit a mix of bacteria and eukaryotes, suggesting that the RNA library was contaminated.
Gene Models | FilteredModels3 | |
length (bp) of: | average | median |
gene | 8068 | 5061 |
transcript | 1487 | 1115 |
exon | 262 | 149 |
intron | 1412 | 830 |
description: | ||
protein length (aa) | 403 | 281 |
exons per gene | 5.67 | 4 |
# of gene models | 25012 |
Collaborators
- Scott Lindell at Woods Hole Oceanographic Insitution
- Sergey Nuzhdin at University of Southern California, CA
Links
- JGI Algae EcoGroup Portal
- JGI PhyloGroup Portals: Heterokonta Ochrophyta Phaeophyta