Info • Saccharina latissima SL-CT1-FG3 v1.0

Status

[June 2021] The Saccharina latissima SL-CT1-FG3 v1.0 genome was sequenced with PacBio and assembled with HiFiAsm by the HudsonAlpha Genome Sequencing Center. The resulting assembly was polished using RACON, scaffolded using HiC data, and screened for contaminants. Aquatic genomes can often harbor contaminants that are difficult to identify, so Illumina depth was employed to assist in contaminant screening. The transcriptome was sequenced with Illumina by HudsonAlpha, and then assembled with Trinity by the Joint Genome Institute (JGI). Subsequently, the JGI Annotation Pipeline was used to generate structural and functional annotations. Additional screening of contaminants using NCBI's FCS_GX and vector screening identified an additional 218 gene models that were excluded from FilteredModels3 to produce the default analysis track ("FrozenGeneCatalog_20240409"). The mitochondrial and plastid genomes were assembled separately, annotated by the Nuzhdin lab using the GeSeq MPI-MP Chlorobox program, and are available in the Downloads page.

Genome Assembly
Genome Assembly size (Mbp) 615.55
Sequencing read coverage depth 185.23x
# of contigs 1513
# of scaffolds 1180
# of scaffolds >= 2Kbp 1180
Scaffold N50 109
Scaffold L50 (Mbp) 1.35
# of gaps 333
% of scaffold length in gaps 0.5%
Three largest Scaffolds (Mbp) 11.32, 8.81, 7.48


ESTs Data set # sequences total # mapped to genome % mapped to genome
Ests est.fasta 571770431 443028122 77.5%
Other JGI_RNA_contigs 503924 120242 23.9%
  • Please note: Using BLAST, unmapped RNA contigs were found to hit a mix of bacteria and eukaryotes, suggesting that the RNA library was contaminated.

Gene Models FilteredModels3
length (bp) of: average median
gene 8068 5061
transcript 1487 1115
exon 262 149
intron 1412 830
description:
protein length (aa) 403 281
exons per gene 5.67 4
# of gene models 25012


Collaborators

Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.